Golgi method for old formol-fixed human foetal tissue.

Golgi method for old formol-fixed human foetal tissue.

The Golgi method can be successfully applied to human embryonic and foetal nervous tissues which have been fixed in formol for years provided the scalp and skull of the specimen were opened originally for proper fixation. The following method is a variation of the Golgi method which has proved successful: 1 Wash the complete embryo or foetus (up to the age of 12 weeks-crown-rump length 87 mm) i...

Differentiation of foetal and maternal erythrocytes in formol-fixed tissues.

A Convenient Method for the Formol Titration

The formol titration, as described by Srrensen, 1 has been most useful in the determination of amino-acids and especially in following the course of hydrolysis of proteins. The method as originally described could not be used for accurate determinations of small quantifies of amino-acids owing to the difficulty of determining the exact end-point. The value obtained also depends on the point at ...

Bone tissue engineering using foetal cell therapy.

Different cell sources for bone tissue engineering are reviewed. In particular, adult cell source strategies have been based on the implantation of unfractionated fresh bone marrow; purified, culture expanded mesenchymal stem cells, differentiated osteoblasts, or cells that have been modified genetically to express rhBMP. Several limiting factors are mentioned for these strategies such as low n...

Lagrangian Relaxation Method for the Step fixed-charge Transportation Problem

In this paper, a step fixed charge transportation problem is developed where the products are sent from the sources to the destinations in existence of both unit and step fixed-charges. The proposed model determines the amount of products in the existing routes with the aim of minimizing the total cost (sum of unit and step fixed-charges) to satisfy the demand of each customer. As the problem i...